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plasmid d1er  (Thermo Fisher)


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    Structured Review

    Thermo Fisher plasmid d1er
    Plasmid D1er, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/d1er+plasmid/plasmid+d1er/pm38513662-850-8-15
    Average 90 stars, based on 1 article reviews
    plasmid d1er - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: APOL1-mediated monovalent cation transport contributes to APOL1-mediated podocytopathy in kidney disease
    Article Snippet: .. D1ER plasmid, a genetic coded calcium indicator (GCCI) of SR/ER Ca 2+ , was electroporated using a neon electroporation system (Thermo Fisher) into T-REx-293 G1 cells and seeded on a 35 mM MatTeck plate. .. After treating the cells for 12 hours with Tet (50 ng/mL) or Tet (50 ng/mL) plus VX-147 (3 μM), the plates were mounted to the stage of Nikon TE2000-E inverted microscope and continuously perfused with Tyrode’s solution containing 145 mM NaCl, 5 mM KCl, 10 mM HEPES, 5 mM glucose, 2 mM CaCl 2 , and 0.3 mM NaH 2 PO 4 , 1 mM MgCl 2 , and pH7.4.

    Electroporation:

    Article Title: APOL1-mediated monovalent cation transport contributes to APOL1-mediated podocytopathy in kidney disease
    Article Snippet: .. D1ER plasmid, a genetic coded calcium indicator (GCCI) of SR/ER Ca 2+ , was electroporated using a neon electroporation system (Thermo Fisher) into T-REx-293 G1 cells and seeded on a 35 mM MatTeck plate. .. After treating the cells for 12 hours with Tet (50 ng/mL) or Tet (50 ng/mL) plus VX-147 (3 μM), the plates were mounted to the stage of Nikon TE2000-E inverted microscope and continuously perfused with Tyrode’s solution containing 145 mM NaCl, 5 mM KCl, 10 mM HEPES, 5 mM glucose, 2 mM CaCl 2 , and 0.3 mM NaH 2 PO 4 , 1 mM MgCl 2 , and pH7.4.



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